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Time：2017-02-17Publisher：Xingyuan

Prohibitions in animal derived foods (such as β- The residues of agonists, heavy metals, and excessive levels of biological toxins have become a problem that troubles world food hygiene. These residues not only affect the export trade of livestock and poultry products in China, but also threaten the health of consumers. Many& Nbsp; Using veterinary drug residue monitoring as a technical barrier to prevent the normal export of livestock products in China

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Immunocolloidal gold diagnosis is used to preliminarily test the content of heavy metal ions in agricultural and livestock products, which can save time, accelerate the detection process, and improve the efficiency of on-site sampling and customs clearance detection. Currently, an immunocolloidal gold detection method for pollutants has been established, and this method has been used for the analysis and detection of heavy metal ions. So far, immunocolloidal gold detection technology has been successfully used for the detection of Gu, mercury, Fu, lead, and uranium in water. In terms of toxin detection, colloidal gold test strips have also shown their advantages. The colloidal gold test strips developed by Lai Weihua et al. are used to detect ochratoxin A, with a detection limit of." 10ng/mL. Klewitz et al. used colloidal gold labeling technology to develop test strips for detecting type A, B, D, and E botulinum toxin, with sensitivity even reaching 50pg/mL. In recent years, due to & Nbsp; Strengthening the monitoring of clenbuterol has cracked down on the illegal use of clenbuterol in pig farming. However, in the animal husbandry industry, substitutes for clenbuterol such as ractopamine (RCT) and salbutamol have quietly emerged. Xu Society coupled ractopamine (CRAC) with BSA to produce artificial antigen immune BALB/c: mice. Anti RAC monoclonal antibodies were prepared using hybridoma technology, and RAC colloidal gold immunochromatographic test paper IV was developed using competitive method using colloidal gold immunochromatography technology. The experiment shows that the sensitivity of the test strip is 5ng/mL, and it can be stored for more than 12 months under room temperature dry conditions. The cross reactivity with clenbuterol, adrenaline, norepinephrine, isoproterenol, phenoxypropanol, salbutamol, and dopamine is less than 0.03%. The sensitivity, stability, and specificity of the test strip meet the detection requirements

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