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Time：2017-04-18Publisher：Xingyuan

1. Immunoblotting

Immunoblotting is to transfer the protein bands separated by polypropylene phthalamide gel electrophoresis to the nitrocellulose membrane, and then use enzyme immunoassay (or immunofluorescence, RIA) for quantitative analysis. Similarly, gold labeled cartridge immunocolloidal gold can also be used for immunoblotting. After the transferred nitrocellulose membrane is insulated with specific antibodies, it reacts with colloidal gold sensitized by Staphylococcus aureus and Kammai white to wash away excess colloidal gold. The amount of specific antigen in the sample is determined based on the color of the colloidal gold particles on the membrane

Gold Label Card

Colloidal gold immunoblotting technology is simple and has considerable sensitivity, and has great potential for clinical immunodiagnosis.

2. Immunogold Electron Microscopy

The application of immunogold electron microscopy has further deepened into gene transcription level, gene expression detection, chromosome gene localization, spatial distribution analysis of specific nucleic acid sequences in cells, and qualitative or quantitative analysis during nucleic acid replication. The non isotopic nucleic acid probe technology of colloidal gold labeled anti digoxin antibodies is very useful in nucleic acid determination, PCR product analysis, and in situ hybridization analysis.

. In recent years, the streptavidin colloidal gold probe has made a breakthrough in in in situ hybridization and immunocytochemistry research, not only able to perform DNA sequence ultrastructural level analysis on chromatin and chromosomes& Nbsp; Localization, which can be used for laboratory diagnosis of mammalian tissue growth, related virus genotypes and phenotypes, has become a new type of cytochemical testing reagent with broad application prospects

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