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Time：2017-06-29Publisher：Xingyuan

Foot and mouth disease (FMD) is classified as Class A by the World Organization for Animal Health (OIE)& Nbsp;. The pathogen of foot-and-mouth disease virus (FMDV), type O, is showing an epidemic trend, followed by type A and Asia I. Jiang Tao et al. established a standardized diagnostic colloidal gold immunochromatographic method for detecting foot-and-mouth disease virus types O, A, and Asia I. Purified gold standard strains O, A, Asia type I foot-and-mouth disease antibodies, and sheep anti mouse antibodies were coated on cellulose membranes as detection bands and quality control bands, respectively, and assembled into a standardized diagnostic kit. The results showed that the developed test strip had good sensitivity and could detect a viral dose of 78000 LD50 (1:128 times diluted mouse venom). At the same time, three repeated tests were conducted on positive and negative samples, and the results were the same, indicating good reproducibility. In terms of specificity, pathogens with similar clinical symptoms to foot-and-mouth disease, such as porcine water scar disease virus, vesicular stomatitis virus, and vesicular rash virus, have no cross reactivity. And the Asia I foot-and-mouth disease virus colloidal gold established by Lin Tong et al.

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The test strip does not cross react with foot-and-mouth disease virus types A, O, C, and porcine water scar disease virus, and has good specificity. The compliance rate with other traditional diagnostic methods is 98.8%.

In China, porcine rotavirus infection is quite common. The low detection limit of the A group porcine rotavirus colloidal gold immunochromatographic test strip established by Liu Zhaolei et al. is 1:32 diluted virus culture medium. The repeated detection results of different batches of gold label cartridge test strips are not different, and they are consistent with porcine infectious gastroenteritis virus and flowing diarrhea.

The virus does not undergo cross reactivity and has good specificity. Avian influenza (AI) and Newcastle disease (ND) are two important avian infectious diseases worldwide. Traditional detection methods are time-consuming or require instruments and personnel, which is not conducive to meeting the requirements of on-site testing. Peng Fuhu used the double antibody sandwich method to develop colloidal gold immunochromatographic detection and differential diagnostic test strips for H5 and H9 subtypes of avian influenza virus, as well as colloidal gold immunochromatographic detection and diagnostic test strips for avian influenza and Newcastle disease viruses. The results showed that the developed gold standard cartridge test strips did not react with other avian viruses and had strong specificity. At the same time, a method of coating multiple antibodies on a single membrane was used to establish a differential diagnosis test strip for AIV and NDV, which also has strong specificity and sensitivity, and can be used for the detection and differential diagnosis of AIV and NDV. Xu Yiming and others have developed colloidal gold diagnostic strips for H3 and H7 subtypes of avian influenza viruses. Prepare gold labeled antibodies using monoclonal antibodies against H3 and H7 subtypes. Purified horse anti H3 and H7 subtype AIV polyclonal antibodies and goat anti mouse secondary antibodies were coated on nitrocellulose membrane (NC) as detection and quality control lines, respectively, to prepare colloidal gold test strips. After standard positive antigen testing, the results are consistent with the methods of hemagglutination test, hemagglutination test, AC-ELISA, and RT-PCR. In terms of specificity, there was no cross reaction with the H5 and H9 subtypes of AIV standard antigens, disease materials, infectious and Newcastle disease antigens, indicating good specificity

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