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NEWSColloidal gold immunoassay (GICA) refers to a solid-phase membrane immunoassay technique that combines colloidal gold labeling, immunoassay, and protein chromatography techniques, using nitric acid fiber membranes as carriers. The principle is to use nitrocellulose membrane as a solid-phase carrier, and the sample solution swims on the chromatographic strip through capillary action; At the same time, the analyte in the sample undergoes a highly specific and affinity immune reaction with the receptor (such as antibody or antigen) of the analyte on the chromatography material. During the chromatography process, the immune complex is enriched or intercepted in the region (detection band) of the chromatography material, and the experimental results are intuitive through the aggregation of colloidal gold particles that can be directly observed in a short period of time (5-10 minutes). It does not require the separation of bound and free markers, so there are no complicated sampling and washing steps like in Elisa's experiment. This technology has the characteristics of simple operation, clear and intuitive detection results, and does not require instruments (or only simple instruments), making it particularly suitable for on-site testing
Gold standard cartridgeColloidal gold immunochromatography can be divided into two categories based on the different molecular sizes of the analytes: double antibody sandwich method and competitive method. Double antibody sandwich method is suitable for detecting large molecule substances with strong intuitiveness, while competitive method is suitable for detecting small molecule substances. With the large-scale and intensive development of China's aquaculture industry, the harm of animal contagiousness to the industry is gradually increasing. Therefore, how to achieve detection for timely prevention and control has become a key link. The emergence of gold standard cartridge colloidal gold chromatography technology makes on-site detection possible."